The effects of in ovo injection with sulfur amino acids and folic acid on the gene expression, relative organ weights, hematologic parameters, performance, and carcass characteristics of broiler chickens.

This study aimed to evaluate the effect of in ovo injection of folic acid (FA) and sulfur amino acids (SAAs) on the mitotic activity of myocytes, performance, relative organ weight, hematological values, and characteristics of broiler chicken carcasses. A total of 1200 fertile eggs from 42-week-old Ross AP© breeders were inoculated in the albumen on the first day of incubation in a completely randomized design with one of the treatments: C-intact eggs; SS: inoculation with 0.5 mL of saline solution; FA: 0.150 mg of FA; SAA: 5.90 mg of L-methionine and 3.40 mg of L-cysteine; or FA/SAA: FA + SAA. The inoculation of SAA did not influence (p > 0.05) the post-hatching characteristics of the chickens. FA inoculation increased (p < 0.05) the expression of the PAX7 and MYF genes in the pectoralis muscle of hatched chicks and reduced (p < 0.05) feed conversion at 42 days of age. The combination of SAA + FA increased (p < 0.05) the depth of the ileal crypt on the 1st day after hatching and the relative weight of the spleen and thymus on the 21st day of life. In conclusion, the inoculation of FA on the 1st day of incubation increases gene expression and improves the performance of broilers.

Dietary crude protein levels during growth phase affects reproductive characteristics but not reproductive efficiency of adult male Japanese quails.

OBJECTIVE: The objective was to evaluate the influence of different dietary crude protein (CP) levels during the growth phase on reproductive characteristics and reproductive efficiency as well as the body development of adult male Japanese quail. METHODS: Three hundred one-day-old male quails were distributed into five treatments with diets containing different CP levels (18%, 20%, 22%, 24%, and 26%) in a completely randomized design, with six replicates of ten birds each. The CP diets were applied only during the growth phase (1 to 35 days). At 36 days of age, the birds were transferred to 30 laying cages with three males and nine females each, and all birds received the same diet formulated to meet production-phase requirements until 96 days of age. RESULTS: The growth rate of the birds increased linearly (p<0.01) with increasing dietary CP, but the age of maximum growth decreased (p<0.05). At growth maturity, all birds had the same body weight (p>0.05). At 35 days of age, higher weight gain was obtained (p<0.05) with diets containing 22% CP or higher. No effects on feed conversion were observed in this phase. The increase in dietary CP enhanced (p<0.01) nitrogen intake and nitrogen excretion but did not affect (p>0.05) nitrogen retention. Testis size, seminiferous tubular area, number of spermatogonia, and germinal epithelial height at 35 days of age increased linearly (p<0.05) with dietary CP, while the number of Leydig cells decreased (p<0.01). The Sertoli cell number at 60 days of age increased linearly (p<0.01) with dietary CP. Dietary CP levels did not affect cloacal gland size, foam weight, foam protein concentration, semen volume, or flock fertility at 90 days of age. CONCLUSION: Dietary CP concentration affected body and testicular development in male Japanese quails but did not affect reproductive efficiency.

Use of Tribulus terrestris and Lepidium meyenii extract in rats: reproductive, biochemical and body parameters.

The oral administration of Tribulus terrestris and Lepidium meyenii extracts on reproductive, biochemical and body parameters was evaluated in rats. Thirty-six male Wistar rats weighting 210 ± 18 g were divided into six experimental groups (n = 6). Each group received, daily for 28 days, different solutions: T. terrestris (100 mg/kg), L. meyenii (1 g/kg) and T. terrestris at doses of 75, 50 and 25 mg/kg combined with L. meyenii at doses of 0.25, 0.5 and 0.75 g/kg, respectively, and distilled water (control). T. terrestris increased (p < 0.05) the serum testosterone, regardless of dose. Combined use of the extracts increased (p < 0.05) the diameter of the epididymal duct and epididymis lumen. The combinations of T. terrestris (75 and 50 mg/kg) with L. meyenii increased (p < 0.05) the sperm concentration. There were no differences (p > 0.05) in the other semen characteristics; relative weight of organs; and serum levels of urea, creatinine, alanine and aspartate transaminase, gamma glutamyl transferase, cholesterol, triglycerides, glucose, follicle-stimulating hormone and luteinizing hormone. No histopathological changes were observed (p > 0.05). It is concluded that the association of T. terrestris and L. meyenii has positive effects on serum testosterone, sperm concentration and epididymal morphology, with no evidence of effects in the testis, liver, spleen and kidneys.

Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency.

This work sought to address whether the presence of exogenous bicarbonate is required for pig sperm to elicit in vitro capacitation and further progesterone-induced acrosome exocytosis. For this purpose, sperm were either incubated in a standard in vitro capacitation medium or a similar medium with different concentrations of bicarbonate (either 0 mM, 5 mM, 15 mM or 38 mM) and BSA (either 0 mg/mL or 5 mg/mL). The achievement of in vitro capacitation and progesterone-induced acrosomal exocytosis was tested through the analysis of sperm motility, plasma membrane integrity and lipid disorder, acrosome exocytosis, intracellular calcium levels, mitochondria membrane potential, O2 consumption rate and the activities of both glycogen synthase kinase 3 alpha (GSK3α) and protein kinase A (PKA). While sperm incubated in media without BSA or BSA/bicarbonate, they did not achieve in vitro capacitation; those incubated in media with BSA achieved the capacitated status under any bicarbonate concentration, even when bicarbonate was absent. Moreover, there were differences related to the concentration of bicarbonate, since sperm incubated in media with BSA and with no bicarbonate or 5 mM bicarbonate showed lower overall efficiency in achieving in vitro capacitation than those incubated in the presence of BSA and 15 mM or 38 mM bicarbonate. Additionally, at the end of the experiment, sperm incubated in the presence of BSA and 38 mM bicarbonate showed significantly (p < 0.05) lower values of motility and plasma membrane integrity than those incubated in media with BSA and lower concentrations of bicarbonate. In conclusion, BSA is instrumental for pig sperm to elicit in vitro capacitation and trigger the subsequent progesterone-induced acrosome exocytosis. Furthermore, while exogenous bicarbonate does not seem to be essential to launch sperm capacitation, it does modulate its efficiency.

Dose-response effect of prebiotic ingestion (ß-glucans isolated from Saccharomyces cerevisiae) in diabetic rats with periodontal disease.

BACKGROUND: Periodontal disease is one of the most frequent comorbidities in diabetic patients and can contribute to poor blood glucose control. OBJECTIVE: To evaluate the effects of ingesting different doses of beta-glucans (BG) isolated from Saccharomyces cerevisiae on alveolar bone loss (ABL) and inflammatory/metabolic parameters in normal and diabetic rats with ligature-induced periodontal disease (PD). DESIGN: Sixty male rats were assigned into two groups: non-diabetic or diabetic (i.p. 70 mg/kg streptozotocin) with PD. Then, groups were subdivided into five subgroups according BG doses: 0 mg/Kg; 10 mg/Kg; 20 mg/Kg; 40 mg/Kg or 80 mg/Kg. Animals received BG for 28 days and ligatures were placed on lower first molars during the last 14 days. RESULTS: ABL of diabetic and non-diabetic animals receiving BG 40 mg/kg (1.33 ± 0.03 mm and 0.77 ± 0.07 mm, respectively) and 80 mg/kg (1.26 ± 0.07 mm and 0.78 ± 0.05 mm, respectively) doses was lower (p < 0.05) in comparison to respective controls (1.59 ± 0.11 mm and 0.90 mm ±0.08). COX-2 (Control: 1.66 ± 0.12; 40 mg/kg: 1.13 ± 0.07; 80 mg/kg: 0.92 ± 0.18) and RANKL expressions (Control: 1.74 ± 0.34; 40 mg/kg: 1.03 ± 0.29 ;80 mg/kg: 0.75 ± 0.21), together with the RANKL/OPG ratio (Control: 1.17 ± 0.08; 40 mg/kg: 0.67 ± 0.09; 80 mg/kg: 0.63 ± 0.28) were attenuated above the same dose (p < 0.05). BG did not influence (p > 0.05) metabolic parameters in non-diabetic rats. In diabetic animals, doses above 40 mg/kg reduced IL-1ß (Control: 387 ± 66; 40 mg/kg: 309 ± 27; 80 mg/kg: 300 ± 14) and TNF-α (Control: 229 ± 19; 40 mg/kg: 128 ± 53; 80 mg/kg: 71 ± 25), blood glucose levels (Control: 402 ± 49; 40 mg/kg: 334 ± 32; 80 mg/kg: 287 ± 56), total cholesterol (Control: 124 ± 8; 40 mg/kg: 120 ± 10; 80 mg/kg: 108 ± 9), LDL-c + VLDL-c (Control: 106 ± 8; 40 mg/kg: 103 ± 10; 80 mg/kg: 87 ± 10) and triacylglycerols (Control: 508 ± 90; 40 mg/kg: 301 ± 40; 80 mg/kg: 208 ± 61), whereas increased HDL-c (Control: 18 ± 0.5; 40 mg/kg: 19 ± 1; 80 mg/kg: 21 ± 1) (p < 0.05). Optimal dose needed to reduce ABL was higher in diabetic animals with PD. CONCLUSIONS: BG ingestion reduced ABL and improved inflammatory profile in a dose-dependent manner. Best effects were achieved with doses above 40 mg/kg.

Reproductive aspects of Japanese quails (Coturnix coturnix japonica) hatched from eggs incubated under different light colors.

The objective of this study was to evaluate the effect of exposure to different colors of light during egg incubation on the reproductive parameters of male and female Japanese quails. A total of 1776 eggs were incubated under four lighting conditions for 24 h a day during the entire incubation period: white LEDs, green LEDs, red LEDs and darkness (control). The experimental design was a randomized block (incubation time) with four treatments of six replicates of two cages each. After hatching, the birds were housed in brood cages with 18 birds each to 35 days of age, when they were sexed and transferred to production cages for evaluation of reproductive parameters. After the onset of laying, the number of eggs in each cage was recorded daily, and the values were used to obtain the age of the females at first egg and at 80% laying. At 35 and 60 days of age, several birds from each cage were euthanized for anatomical and histological evaluation of the gonads. Two females from each cage were weighed every three days until 60 days of age to determine the growth curve. After 60 days, eggs from each cage were collected and assessed for external and internal quality. At 70, 74 and 78 days of age, semen collection was performed and seminal quality was evaluated. Then, the males were transferred to cages containing 9 females for the fertility test. Hatchability was higher (P < 0.05) in eggs incubated in the dark and under the red LED. The age of maximum growth was higher (P < 0.05) in birds from eggs incubated in the dark and under the white LED. There was no difference (P > 0.05) in the anatomical and histological characteristics of the testicles between the groups incubated under different light colors, except for the diameter of the seminiferous tubules, which was greater (P = 0.05) in the dark and in the white LED groups. There was no effect (P > 0.05) of light color during incubation on the productive index or egg quality of adult birds. There was also no effect (P > 0.05) on sperm quality, except for sperm motility, the values of which were higher (P < 0.05) in birds from eggs incubated in different colors of light. However, this difference was not sufficient to significantly (P > 0.05) influence bird fertility. It is concluded that under the studied conditions, the incubation of quail eggs under white, red, and green LED lamps does not influence the reproductive characteristics of the quails.

Histone deacetylase inhibitor during in vitro maturation decreases developmental capacity of bovine oocytes.

This study aimed to evaluate the effect of scriptaid during pre-maturation (PIVM) and/or maturation (IVM) on developmental competence of bovine oocytes. Cumulus-oocyte complexes (COCs) were submitted to PIVM for 6 h in the presence or absence of scriptaid. COCs were distributed into five groups: T1-IVM for 22 h, T2-PIVM for 6 h and IVM for 22 h, T3-PIVM with scriptaid for 6 h and IVM for 22 h, T4-PIVM for 6 h and IVM with scriptaid for 22 h, and T5-PIVM with scriptaid for 6 h and IVM with scriptaid for 22 h. Nuclear maturation, gene expression, cumulus cells (CCs) expansion, and embryo development and quality were evaluated. At the end of maturation, all groups presented the majority of oocytes in MII (P>0.05). Only HAT1 gene was differentially expressed (P<0.01) in oocytes with different treatments. Regarding embryo development at D7, T4 (23%) and T5 (18%) had lower blastocyst rate (P<0.05) than the other treatments (T1 = 35%, T2 = 37% and T3 = 32%). No effect was observed when scriptaid in PIVM was used in less competent oocytes (P>0.05). In conclusion, presence of scriptaid in PIVM and/or IVM did not improve developmental competence or embryo quality.

Antioxidant enrichment of rooster semen extenders - A systematic review.

The purpose of this systematic review was to evaluate the potential benefits of antioxidant enrichment of semen extenders. These substances are used to combat oxidative stress during processing and conservation of rooster semen. A literature search was performed in June 2020 using the keywords rooster AND (semen OR spermatozoa OR sperm OR ejaculate OR ejaculation). This report followed the PRISMA (Preferred Reporting Items for Systematic Review and Meta-Analysis) guidelines. The PICO (population intervention comparison outcome) question was defined to compare roosters (Population) which had added antioxidants in the semen (Intervention) compared to the no-antioxidant group (Control); the outcome was semen quality (Outcome). Only articles investigating rooster cooled or frozen enriched semen with antioxidant extenders (Gallus Gallus domesticus) were selected by reading the title and abstract, totalizing 38 articles. After full text reading, we found that only 13 studies carried out sperm characteristics and fertility assays. To assess article quality, 15 items related to rooster breeding conditions, seminal collection methodology, and analyzed variables (seminal characteristics and fertility test) were established. There were positive effects of antioxidants on the preservation of seminal characteristics (motility, viability, membrane integrity, antioxidant activity, and lipid peroxidation) and on semen fertility after the conservation process. We conclude that the antioxidants reduce the oxidative stress and improve fertilizing capacity. The most used substances for cooled semen are glutathione, CoQ10, and l-carnitine; whereas for frozen semen, resveratrol, lycopene, and quercitin are most frequently used.

Chlorogenic acid improves the quality of boar semen processed in Percoll.

This study aimed to evaluate if the addition of chlorogenic acid (ChA) to semen extenders improves the quality of cooled boar semen processed in Percoll. The experimental design was randomized blocks (ejaculates) in a 2×3 factorial (with or without Percoll, and three antioxidant systems: a negative control, without supplementation, a positive control - vitamin E, and ChA), totaling six treatments and 12 repetitions. ChA and vitamin E (VE) were added at 4.5 mg/ml and 400 µg/ml in extender, respectively. At 0, 48 and 72h of storage at 15ºC, 80 ml insemination doses each containing 2.0 billion sperm cells were submitted to centrifugation in Percoll. The use of Percoll impaired (P<0.01) all motility patterns but decreased (P<0.01) the number of abnormal cells at 0, 48 and 72h of storage. Both VE and ChA improved (P<0.05) the total motility after Percoll processing, but only in semen stored for 48h. The same effect was not observed (P>0.05) in semen stored for 72h. ChA improved (P<0.05) the total motility of the semen stored for 72h, but this effect was not observed (P>0.05) when the semen was processed in Percoll. The antioxidants had no effect (P>0.05) on the viability and integrity of the acrosome, but ChA reduced (P<0.05) the number of abnormal cells at 0h, while VE increased the number of abnormal cells in semen stored for 72h, independent of the use of Percoll. There was no effect (P>0.05) of antioxidants or Percoll on the concentration of malondialdehyde in seminal plasma. The use of Percoll had no effect (P>0.05) on the cholesterol efflux, but ChA increased (P<0.05) this parameter at 0h and reduced (P<0.05) in the semen stored for 72h not processed with Percoll. In conclusion, the addition of ChA to semen extenders improved the quality of boar semen processed or not in Percoll.

Occupational exposure to Brucella spp.: A systematic review and meta-analysis.

Brucellosis is a neglected zoonotic disease of remarkable importance worldwide. The focus of this systematic review was to investigate occupational brucellosis and to identify the main infection risks for each group exposed to the pathogen. Seven databases were used to identify papers related to occupational brucellosis: CABI, Cochrane, Pubmed, Scielo, Science Direct, Scopus and Web of Science. The search resulted in 6123 studies, of which 63 were selected using the quality assessment tools guided from National Institutes of Health (NIH) and Case Report Guidelines (CARE). Five different job-related groups were considered greatly exposed to the disease: rural workers, abattoir workers, veterinarians and veterinary assistants, laboratory workers and hunters. The main risk factors and exposure sources involved in the occupational infection observed from the analysis of the articles were direct contact with animal fluids, failure to comply with the use of personal protective equipment, accidental exposure to live attenuated anti-brucellosis vaccines and non-compliance with biosafety standards. Brucella species frequently isolated from job-related infection were Brucella melitensis, Brucella abortus, Brucella suis and Brucella canis. In addition, a meta-analysis was performed using the case-control studies and demonstrated that animal breeders, laboratory workers and abattoir workers have 3.47 [95% confidence interval (CI); 1.47-8.19] times more chance to become infected with Brucella spp. than others individuals that have no contact with the possible sources of infection. This systematic review improved the understanding of the epidemiology of brucellosis as an occupational disease. Rural workers, abattoir workers, veterinarians, laboratory workers and hunters were the groups more exposed to occupational Brucella spp. infection. Moreover, it was observed that the lack of knowledge about brucellosis among frequently exposed professionals, in addition to some behaviors, such as negligence in the use of individual and collective protective measures, increases the probability of infection.

In ovo injection with glycerol and insulin-like growth factor (IGF-I): hatchability, intestinal morphometry, performance, and carcass characteristics of broilers.

The objective of this study was to evaluate the effects of in ovo injection with glycerol (GLY) and insulin-like growth factor (IGF-I) on hatchability, biochemical parameters, intestinal morphometry, performance, and carcass characteristics of broiler chickens. A total of 400 fertilised eggs were distributed into five experimental groups. The treatments were arranged as non-injected (control), saline solution injected (0.9% NaCl solution), GLY solution injected (10 nmol/ml), IGF-I solution injected (100 ng/ml), and GLY + IGF-I solution injected. At 17.5 d of incubation, 0.5 ml of each solution was injected into the amniotic fluid of each egg of the injected groups. The injection of different solutions did not influence the hatchability and incubation time of the eggs. Compared to intact eggs, IGF-I and IGF-I+ GLY increased (p < 0.01) the blood IGF-I at hatching. Higher hepatic glycogen was observed (p < 0.05) with GLY or IGF-I. The tested substances decreased (p = 0.02) the fructose 1,6-biphosfate phosphatase activity but did not affect glycaemia. No difference in performance was observed in the first week. Higher feed intake and weight gain with lower feed conversion ratio was obtained ( p  < 0.05) with IGF-I at 14 d. At 21 d, higher weight gain was obtained (p = 0.05) with IGF-I, GLY, IGF-I, and GLY + IGF-I, resulting (p < 0.01) in birds with greater weight gain at 35 and 42 d of age. GLY provided higher villus height in the ileum at hatching and at 7 d of age. The tested solutions increased the relative weight of the liver at hatching. At 42 d of age, no carcass characteristics were influenced. It is concluded that GLY and IGF-I, together or separately, can be used in the in ovo feeding to improve the post-hatch performance of broilers, without affecting hatchability and carcass composition.

Addition of insulin-like growth factor I (IGF-I) and reduced glutathione (GSH) to cryopreserved boar semen.

The objective of this study was to evaluate the effect of adding reduced glutathione (GSH) to a boar semen freezing extender supplemented with insulin-like growth factor I (IGF-I) or anti-IGF-I. Eight ejaculates from eight boars were extended to obtain insemination doses, which were supplemented with either recombinant human IGF-I (30 ng/mL) or anti-IGF-I (60 ng/mL) shortly after extension. After 24 h of liquid storage at 17 °C, the semen was frozen with or without GSH (5 mM) in the freezing extender for a total of six treatments. Osmotic resistance and acrosome integrity was greater in fresh semen (P <  0.05) soon after adding IGF-I or the anti-IGF-I antibody. After 24 h of cooling, the supplementation with these compounds resulted in an increased (P <  0.05) percentage of sperm with relatively greater mitochondrial activity and reduced the percentage of cells with relatively greater concentrations of superoxide. After thawing, there was a reduction (P <  0.05) in the percentage and fluorescence intensity of sperm with greater quantities of superoxide and peroxide only in samples treated with GSH + IGF-I and GSH + anti-IGF-I. The addition of GSH (alone or in combination with IGF-I or anti-IGF-I), however, reduced the percentage of sperm with an intact acrosome (P < 0.05). The same effect was not observed with IGF-I or anti-IGF-I alone. In conclusion, the addition of IGF-I or anti-IGF-I improved the quality of fresh or liquid-stored semen. Using GSH in the freezing extender improved the antioxidant potential of frozen semen only in combination with IGF-I or an anti-IGF-I antibody.

Reproductive Characteristics of Cockatiels (Nymphicus hollandicus) Maintained in Captivityand Receiving Madagascar Cockroach (Gromphadorhina portentosa) Meal.

The aim of this study was to evaluate the use of a Madagascar cockroach (Gromphadorhina portentosa) meal in the feed of cockatiels (Nymphicus hollandicus) in captivity and its influence on the reproductive characteristics of these birds. Twelve pairs of birds were used during two subsequent reproductive cycles of 130 days each, with time divided into four phases: laying, incubation, rearing of chicks until 30 days of age, and return to the new laying phase. The pairs were divided into two groups: a control group, which received a commercial diet for psittacines + a mixture of seeds, and a test group, which received the same diet as the control group except for the addition of a Madagascar cockroach meal in a ratio of 14 g of commercial food to 1 g of cockroach meal (6.6%). After hatching, chicks remained with their parents until 30 days of age. Subsequently, the chicks were transferred to another room and monitored until the 90th day of life. The inclusion of cockroach meal did not influence (p > 0.05) the intake of commercial food and mixture of seeds during the reproductive phases evaluated, except for feed intake, which was increased relative to control values (p = 0.02) in the return-to-laying phase. Yolk cholesterol content, egg width and egg shape index were increased with the inclusion of the cockroach meal, whereas the number of days to return to the new laying phase was reduced compared to the control (p = 0.02). The number of eggs laid decreased (p < 0.05) with the inclusion of the cockroach meal; however, the percentage of hatching was higher in the test group than in the control group (p < 0.05). No significant effect (p > 0.05) of dietary treatment was observed on the number of viable chicks at 1, 30 and 90 days of age or on the contents of most fatty acids present in the yolk. The findings of this study indicate that a Madagascar cockroach meal can be used as an alternative feedstuff in the diets for cockatiels and can lead to minor improvements in reproductive characteristics when replacing 6.6% of the commercial pelleted diet.

Smartphone use while eating increases caloric ingestion.

The use of smartphones during meals may possibly influence the number of ingested calories. We evaluated the influence of smartphones' distraction during eating on caloric intake. Physical (masticatory parameters, sex and body mass index - BMI); environmental (with or without distraction) and psychological (stress levels) variables were evaluated as confounding factors. Sixty-two adults were recruited for experimental snack tests performed on four different days. At baseline, we evaluated masticatory performance, swallowing threshold, masticatory frequency and body mass index (BMI). In the following three sessions, volunteers were presented a snack test in which participants ate under three experimental conditions: no distraction, using their smartphones, or reading a printed text. At the end of each session, total and nutritional compound stratified caloric intake was measured. Three-way mixed model ANOVA was used to test the effect of experimental condition on the total, carbohydrate and lipid caloric intakes. Hierarchical multiple linear regression models were used to estimate the influence of confounding factors on the total caloric intake. We found an effect of the condition (no distraction, smartphone or reading) on the total caloric (p = .007) and lipid intake (p = .002). When eating without distraction, the mean caloric intake was 535 (±164) kcal, in comparison to 591 (±203) kcal using smartphone (p = .05) and 622 (±226) kcal (p = .002) reading a text (no significant difference between distracters). Eating with distracters increased approximately 15% caloric ingestion. An interaction condition*sex effect was observed on lipid intake (p = .020). Energy intake was found to be dependent on sex and age, in which older men ingested more calories. Smartphone use during a meal increased caloric and lipid intake, depending on sex and age in young adults with complete dentition.

Oat beta-glucan as a dietary supplement for dogs.

The aim of this study was to evaluate the effects of oat beta-glucan supplementation on metabolic, physiological, immunological and nutritional variables in adult dogs. Fourteen dogs were fed a control diet or a diet supplemented with 1% beta-glucan during 71 days. Serum concentrations of glucose, total cholesterol and lipoprotein fractions, as well as plasma concentrations of peptide YY and ghrelin, were determined. In addition, coefficient of total tract apparent macronutrient digestibility (CTTAD), food intake and fecal output, score, and pH were evaluated. For evaluation of immunological variables, serum concentrations of interleukin-4 and interferon gamma were determined on days 0, 57 and 71, which corresponded to blood collection prior to beta-glucan supplementation, and at 7 and 14 days post first and second dose of vaccination (Pneumodog®, Merial, Campinas, Brazil), respectively. Animals fed the supplemented diet showed (P<0.05) lower serum concentrations of total cholesterol and low and very low density lipoproteins, lower coefficients of total tract apparent digestibility of dry matter, organic matter, mineral matter and ether extract, higher fecal output and lower fecal consistency, as well as a tendency (P = 0.07) of decreasing the coefficient of total tract apparent digestibility of crude protein. Moreover, the supplemented diet resulted in lower number of red blood cells, hematocrit percentage and hemoglobin concentration 21 days post-vaccination as well as lower serum concentration of interleukin-4 seven days post-vaccination (P<0.05). It is concluded that oat beta-glucan extract can be used as a dietary supplement for dogs at a dose of 10 g/kg of food, being effective in reducing blood concentrations of total cholesterol, LDL-c and VLDL-c as well as CTTAD of nutrients, demonstrating a potential to be used in the feeding of obese animals. In addition, by reducing the predominance of a Th2 response, oat beta-glucan can positively modulate the vaccine response of animals.

Supplementing Maturation Medium With Insulin Growth Factor I and Vitrification-Warming Solutions With Reduced Glutathione Enhances Survival Rates and Development Ability of in vitro Matured Vitrified-Warmed Pig Oocytes.

The present study sought to determine whether in vitro maturation (IVM) of pig oocytes in a medium supplemented with insulin growth factor-I (IGF-I) and subsequent vitrification with or without reduced glutathione (GSH) affect their quality and developmental competence, and the expression of genes involved in antioxidant, apoptotic and stress responses. In Experiment 1, cumulus-oocyte complexes were matured in the absence or presence of IGF-I (100 ng·mL-1) and then vitrified-warmed with or without 2 mM of GSH. Maturation rate was evaluated before vitrification, and oocyte viability, DNA fragmentation and relative transcript abundances of BCL-2-associated X protein (BAX), BCL2-like1 (BCL2L1), heat shock protein 70 (HSPA1A), glutathione peroxidase 1 (GPX1) and superoxide dismutase 1 (SOD1) genes were assessed in fresh and vitrified-warmed oocytes. In Experiment 2, fresh and vitrified-warmed oocytes were in vitro fertilized and their developmental competence determined. Whereas the addition of IGF-I to maturation medium had no effect on oocyte maturation, it caused an increase in the survival rate of vitrified-warmed oocytes. This effect was accompanied by a concomitant augment in the relative transcript abundance of HSPA1A and a decrease of BAX. Furthermore, the addition of GSH to vitrification-warming media increased survival rates at post-warming. Likewise, the action of GSH was concomitant with an increase in the relative abundance of GPX1 and a decrease of BAX transcript. Blastocyst rates of vitrified-warmed oocytes did not differ from their fresh counterparts when IGF-I and GSH were combined. In conclusion, supplementing maturation medium with 100 ng·mL-1 IGF-I and vitrification-warming solutions with 2 mM GSH improves the quality and cryotolerance of IVM pig oocytes, through a mechanism that involves BAX, GPX1 and HSPA1A expression.

n-3 Ingestion may modulate the severity of periodontal disease? Systematic review.

The aim of this study was to determine the effects of n-3 ingestion on periodontal disease. Besides, we also investigated the relationship between plasma concentrations of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and/or aracdonic acid (AA), and periodontal disease. An electronic search was performed in several databases with the following keywords: "n-3," DHA, EPA and polynsaturated fatty acids (PUFA) in combination with the term "periodontal disease" (PD). Only studies conducted with humans, involving clinical parameters of PD assessment and use of n-3 were selected, without restriction to the date of publication. The search has returned 1368 articles, 11 of which were selected. The results were separated according to the type of n-3 ingestion: supplementation or n-3 content in normal diet. In the studies where n-3 has been supplemented, there was no significant difference in the clinical severity of PD compared to the control subjects. However, in patients where levels of n-3 were evaluated in a usual diet, a lower disease severity was reported. We have detected a preventive effect related to plasma levels of EPA and DHA against PD progression. Thus, n-3 ingestion may beneficially interfere in PD progression, depending on the duration and dosage of consumption.

Histological characteristics of the gonads of pig fetuses and their relationship with fetal anatomical measurements.

The objective was to evaluate the histomorphometric characteristics of the testis and ovaries of pig fetuses at different gestational ages, as well as their correlation with some fetus measurements. Forty-four fetuses were separated for gender (male and female) and gestational age (50, 80 and 106days of gestation). After slaughter, fetuses had their body length, head and thoracic perimeters measured and their gonads submitted to histomorphometric analyses. The gonadal characteristics at different gestational ages were statistically compared, correlations with the fetal measurements were performed and equations to predict the gonadal characteristics from the fetal measurements were obtained. The testis weight logarithmically increased along pregnancy, whereas ovary weight increased in a linear manner. The cordonal length and number of Sertoli cells were positively correlated with the fetal measurements, being higher at 106days gestation, while the nuclear volume of these cells were negatively correlated. The total number of follicles was higher at day 80 and 106 of pregnancy. The number of oogonia decreased along the pregnancy, however, their nucleus size was increased. The number of follicles and volume of oogonia were positively correlated with the fetal measurements, while the number of oogonia was negatively correlated. Equations were obtained for the prediction of gonadal characteristics of fetuses. We concluded that in pigs testis cell proliferation, ovary development and histological organization was more pronounced during the final third of pregnancy. Fetal weight and size were strongly related to gonadal development, and can be used to estimate the histological characteristics of gonads.

The behavioural study of the bush dog (Speothos venaticus) in captivity with environmental enrichment / Estudo comportamental do cachorro-vinagre (Speothos venaticus) em cativeiro com enriquecimento ambiental

The bush dog (Speothos venaticus) is very rare animal species, whose maintenance and breeding in captivity can induce the expression of atypical behaviour. Information concerning environmental enrichment and assessment models of the behaviour of this species in the literature are insufficient. Therefore, a study was conducted in the Zoo-Botanic Foundation of Belo Horizonte, Minas Gerais, Brazil, to evaluate the behaviour of two male bush dogs kept in captivity with different types of environmental enrichment, in April­May 2012. Data collection was performed before, during and after the presence of the environmental enrichment, three times a week in the morning and afternoon on alternate days for ten minutes, with immediate recording of activity performed by the animal every minute. It was observed that the animals interacted with all three types of ENRI used, but showed a greater interest in ENRI-2. No stereotyped behaviour or aggressive behaviour was observed, suggesting satisfactory welfare conditions for the animals in all environments tested. We conclude that the enrichment protocols can be used to stimulate the natural behaviour of the species, aiding the well-being and quality of life in captivity.

O cachorro-do-mato-vinagre ou cachorro-vinagre (Speothos venaticus) é uma espécie animal bastante rara. Sua manutenção e reprodução em cativeiro pode induzir a expressão de comportamentos atípicos. Informações sobre enriquecimento ambiental e modelos de avaliação do comportamento dessa espécie são insuficientes na literatura. Sendo assim, um estudo foi realizado na Fundação Zoo-Botânica de Belo Horizonte, Minas Gerais, Brasil, para avaliar o comportamento de dois machos de cachorro vinagre mantidos em cativeiro com diferentes tipos de enriquecimento ambiental, entre os meses de abril a maio de 2012. A coleta dos dados foi realizada antes, durante e após a utilização do enriquecimento ambiental, três vezes por semana no período da manhã e da tarde em dias alternados, durante dez minutos, com registro instantâneo da atividade realizada pelo animal a cada minuto. Observou-se que os animais interagiram com os três tipos de enriquecimento utilizados, sendo demonstrado maior interesse pelo ENRI-2. Nenhum comportamento de estereotipia ou comportamento agressivo foi observado, sugerindo condições de bem-estar dos animais em todos os ambientes testados. Conclui-se que os protocolos de enriquecimento podem ser utilizados para estimular o comportamento natural da espécie, auxiliando no bem-estar e qualidade de vida em cativeiro.

Human exposure to Salmonella spp from dog food containing raw meat ­ systematic review / Exposição de humanos à Salmonella spp a partir de alimentos para cães contendo carne crua ­ revisão sistemática

O emprego de dietas para cães contendo carne crua tem sido uma prática popular frequente. Estas, quando contaminadas por Salmonella, podem oferecer risco para a saúde dos animais e dos seres humanos. Este trabalho é uma revisão sistemática da literatura efetuada para avaliar a frequência de dietas para cães que continham carne crua contaminada por Salmonella, e se os cães que as consumiam eliminariam a bactéria em suas fezes. A pesquisa foi realizada em duas bases de dados, Web of Knowledge e PubMed, durante janeiro de 2015. Participaram da pesquisa trabalhos sobre dietas para cães contendo alimentos crus e seu consumo; treze artigos foram selecionados. Em 11 trabalhos foram conduzidas análises microbiológicas para Salmonella nas dietas contendo carne crua, sendo que em quatro desses estudos também foi analisada a presença da bactéria nas fezes dos animais. Em 679 dietas contendo carne crua, 141 delas (20,76%) estavam contaminadas, sendo o sorotipo mais encontrado foi Salmonnela Typhimurium (24,60% do total). Dos estudos que relataram a eliminação da bactéria após o consumo de dietas contendo carne crua, um total de 141 amostras fecais foram analisadas, das quais 73 (51,77%) estavam contaminadas, e o sorotipo mais encontrado foi Salmonella Newport (72,60% do total). Os resultados obtidos permitem afirmar que os proprietários dos cães alimentados por dietas (caseiras ou comerciais) a base de carne crua contaminada estão expostos ao risco de infecção por Salmonella tanto pela manipulação de alimentos como pelas fezes dos animais.(AU)

Feeding dogs raw meat diets is an increasingly popular trend, and when contaminated with Salmonella they may present a risk to the health of both animals and humans. The present investigation is a systematic review of literature to assess the frequency of raw meat diets for dogs contaminated with Salmonella and whether dogs consuming these diets can eliminate it in their faeces. A search was made using two databases: "Web of Knowledge" and "PubMed" in January 2015. Only studies related to raw food diets and consumption by dogs were included. Thirteen articles were selected after refining the search. In 11 studies microbiological analysis was conducted for Salmonella in diets containing raw meat, so that in four of these studies Salmonella in the faeces was also analysed. In the analysis of 679 raw meat diets 141 diets (20.76%) were contaminated with Salmonella, in which the serotype most commonly found was S. Typhimurium (24.60%). Of the studies that reported the number of dogs that eliminated Salmonella in faeces after consumption of diets containing raw meat, 141 faecal samples were analysed, of which 73 (51.77%) were contaminated with Salmonella and the serotype most commonly found was S. Newport (72.60%). The available data suggest that the owners who feed their animals with diets containing contaminated raw meat, either commercial or homemade, are exposed to risk of Salmonella contamination, either by the manipulation of food or by handling the dog's stool.(AU)